Membrane-Permeant, Environment-Sensitive Dyes Generate Biosensors within Living Cells
نویسندگان
چکیده
منابع مشابه
Solvent-sensitive dyes to report protein conformational changes in living cells.
Covalent attachment of solvent-sensitive fluorescent dyes to proteins is a powerful tool for studying protein conformational changes, ligand binding, or posttranslational modifications. We report here new merocyanine dyes that make possible the quantitation of such protein activities in individual living cells. The quantum yield of the new dyes is sharply dependent on solvent polarity or viscos...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2019
ISSN: 0002-7863,1520-5126
DOI: 10.1021/jacs.8b09841